Browse wiki

From MurrayWiki
Jump to: navigation, search
Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo
Abstract Biological signaling systems not only dete
Biological signaling systems not only detect the absolute levels of the signals, but are also able to sense the fold-changes of the signals. The ability to detect fold-changes provides a powerful tool for biological organisms to adapt to the changes in environment. Here we present the first novel syn- thetic fold-change detector (FCD) circuit built from ground up in vivo. We systematically designed the FCD circuit in silico, prototyped it in cell-free transcription-translation platform (TX-TL), and eventually implemented it in E. coli cells. We were able to show that the FCD circuit can not only generate pulse-like behavior in response to input, but also produce the same pulse response with inputs of the same fold-change, despite of di�erent absolute signal levels.
espite of di�erent absolute signal levels.  +
Authors Shaobin Guo, Yutaka Hori, and Richard M Murray  +
Funding Model-guided Discovery and Optimization of Cell-based Sensors +
ID 2014m  +
Source Submitted, 2015 Winter q-bio Conference (5 Nov 2014)  +
Tag ghm15-wqbio  +
Title Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo +
Type Conference Paper  +
Categories Papers
Modification date
This property is a special property in this wiki.
15 May 2016 06:14:37  +
URL
This property is a special property in this wiki.
http://www.cds.caltech.edu/~murray/preprints/ghm15-wqbio_s.pdf  +
hide properties that link here 
Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo + Title
 

 

Enter the name of the page to start browsing from.