Browse wiki
From MurrayWiki
System-level studies of a cell-free transcription-translation platform for metabolic engineering |
Abstract |
Current methods for assembling biosyntheti … Current methods for assembling biosynthetic pathways in microorganisms require a process of repeated trial and error and have long design-build-test cycles. We describe the use of a cell-free transcription-translation (TX-TL) system as a biomolecular breadboard for the rapid engineering of the 1,4-butanediol (BDO) pathway. We demonstrate the reliability of TX-TL as a platform for engineering biological systems by undertaking a careful characterization of its transcription and translation capabilities and provide a detailed analysis of its metabolic output. Using TX-TL to survey the design space of the BDO pathway enables rapid tuning of pathway enzyme expression levels for improved product yield. Leveraging TX-TL to screen enzyme variants for improved catalytic activity accelerates design iterations that can be directly applied to in vivo strain development. tly applied to in vivo strain development. +
|
---|---|
Authors | Yong Y. Wu, Hirokazu Sato, Hongjun Huang, Stephanie J. Culler, Julia Khandurina, Harish Nagarajan, Tae Hoon Yang, Stephen Van Dien, Richard M. Murray + |
Funding | Programmable Molecular Technology Initiative + |
ID | 2017i + |
Source | bioRxiv Technical Report + |
Tag | wu+17-biorxiv + |
Title | System-level studies of a cell-free transcription-translation platform for metabolic engineering + |
Type | Technical report + |
Categories | Papers |
Modification date This property is a special property in this wiki.
|
4 September 2017 05:37:33 + |
URL This property is a special property in this wiki.
|
http://www.biorxiv.org/content/early/2017/08/03/172007 + |
hide properties that link here |
System-level studies of a cell-free transcription-translation platform for metabolic engineering + | Title |
---|