Browse wiki

From MurrayWiki
Jump to: navigation, search
Rapid prototyping of biomolecular circuits through module characterization in cell-free expression systems
Abstract Over the past years, the field of syntheti …
Over the past years, the field of synthetic biology has gained a significant array of tools and parts, making way for increasingly complex bio-molecular circuits to be constructed. The development of biocircuits can be facilitated by assembling parts in a less complex, cell-free, environment which contains only the machinery for gene transcription (TX) and translation (TL), which have been extracted from bacteria. In this project, a part library was collected and used to assemble DNA constructs for a newly designed biocircuit. An in vitro TX-TL extract was used to test the circuit modules using linear DNA, and in parallel with predictive modeling of the biomolecular reactions, the overall circuit design was evaluated. The results have given valuable insight into the performance of the circuit modules in a much shorter time than conventional in vivo cloning and testing would have achieved.
o cloning and testing would have achieved.  +
Authors Anton Frisk  +
ID 2015a  +
Source Masters Thesis, Lund University  +
Tag af15-ms  +
Title Rapid prototyping of biomolecular circuits through module characterization in cell-free expression systems +
Type Masters Thesis  +
Categories Papers
Modification date
This property is a special property in this wiki.
15 May 2016 05:39:28  +
URL
This property is a special property in this wiki.
http://www.antonfrisk.se/wp-content/uploads/2015/05/Frisk.A-Masters-Thesis-204-lossless-FINAL.pdf  +
hide properties that link here 
Rapid prototyping of biomolecular circuits through module characterization in cell-free expression systems + Title
 

 

Enter the name of the page to start browsing from.