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Length and time scales of cell-cell signaling circuits in agar
Abstract A community of genetically heterogeneous c …
A community of genetically heterogeneous cells embedded in an unmixed medium allows for sophisticated operations by retaining spatial differentiation and coordinating division-of-labor. To establish the principles of engineering reliable cell-cell communication in a heterogeneous environment, we examined how circuit parameters and spatial placement affect the range of length and time scales over which simple communication circuits interact. We constructed several "sender" and "receiver" strains with quorum-sensing signaling circuits. The sender cell colony produces acyl homoserine lactones (AHL), which diffuse across the semisolid medium. The receiver cell colony detects these signal molecules and reports by fluorescence. We have found that a single colony of one sender variant is sufficient to induce receiver response at more than 1.5cm separation. Furthermore, AHL degradase expression in receiver colonies produces a signal threshold effect and reduces the response level in subsequent receiver colonies. Finally, our investigation on the spatial placement of colonies gave rise to the design of a multicellular long-range communication array consisting of two alternating colony types. Its signal response successfully propagated colony-by-colony along a six-colony array spanning 4.8cm at a transmission velocity of 12.8 hours per colony or 0.075cm per hour. In addition, we have developed a reaction-diffusion model that recreates the observed behaviors of the many performed experiments using data-informed parameter estimates of signal diffusion, gene expression, and nutrient consumption. These results demonstrate that a mixed community of colonies can enable new patterning programs, and the corresponding model will facilitate the rational design of complex communication networks.
design of complex communication networks.  +
Authors Joy Doong, James Parkin, Richard M. Murray  +
Funding Genetic Circuits for Multi-Cellular Machines +
ID 2017k  +
Source 2018 Winter q-bio  +
Tag dpm18-wqbio  +
Title Length and time scales of cell-cell signaling circuits in agar +
Type Conference paper  +
Categories Papers
Modification date
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14 July 2018 13:59:02  +
URL
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https://www.biorxiv.org/content/early/2017/11/18/220244  +
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Length and time scales of cell-cell signaling circuits in agar + Title
 

 

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