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Design and application of stationary phase combinatorial promoters |
Abstract |
Current bacterial synthetic circuits rely … Current bacterial synthetic circuits rely on the fast dilution and high protein expression that occurs during exponential phase. However, constant exponential phase is both difficult to ensure in a lab environment and almost certainly impractical in any natural setting. Here, we characterize the performance of 13 E. coli native 38 promoters, as well as a previously identified 38 consensus promoter. We then make tetO combinatorial versions of the three strongest promoters to allow for inducible delayed expression. The design of these combinatorial promoters allows for design of circuits with inducible stationary phase activity that can be used for phase-dependent delays in dynamic circuits or spatial partitioning of biofilms. cuits or spatial partitioning of biofilms. +
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Authors | Victoria Hsiao, Aileen Cheng and Richard M. Murray + |
Flags | Biocircuits + |
Funding | Biomolecular Circuits for Rapid Detection and Response to Environmental Events + |
ID | 2016b + |
Source | Submitted, 2016 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference + |
Tag | hcm16-seed + |
Title | Design and application of stationary phase combinatorial promoters + |
Type | Conference paper + |
Categories | Papers |
Modification date This property is a special property in this wiki.
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10 June 2016 05:52:05 + |
URL This property is a special property in this wiki.
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http://www.cds.caltech.edu/~murray/preprints/hcm16-seed_s.pdf + |
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Design and application of stationary phase combinatorial promoters + | Title |
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