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Characterization of minimum inducer separation time for a two-input integrase-based event detector
Abstract In this work, we present modeling and expe
In this work, we present modeling and experimental characterization of the minimum time needed for flipping of a DNA substrate by a two-integrase event detector. The event detector logic di↵erentiates the temporal order of two chemical inducers. We find that bundling biological rate parameters (transcription, translation, DNA search- ing, DNA flipping) into only a few rate constants in a stochastic model is sufficient to accurately predict final DNA states. We show, through time course data in E.coli, that these modeling predictions are reproduced in vivo. We believe this model validation is critical for using integrase-based systems in larger circuits.
ntegrase-based systems in larger circuits.  +
Authors Victoria Hsiao, Yutaka Hori, and Richard M Murray  +
Funding Biomolecular Circuits for Rapid Detection and Response to Environmental Events +
ID 2014m  +
Source Presented, 2015 Winter q-bio Conference (5 Nov 2014)  +
Tag hhm15-wqbio  +
Title Characterization of minimum inducer separation time for a two-input integrase-based event detector +
Type Conference Paper  +
Categories Papers
Modification date
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18 May 2016 02:11:08  +
URL
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http://www.cds.caltech.edu/~murray/preprints/hhm15-wqbio_s.pdf  +
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Characterization of minimum inducer separation time for a two-input integrase-based event detector + Title
 

 

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