Field-Programmable, Recombinase-Based Biomolecular Circuits

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This project funded by the Army's Institute for Collaborative Biotechnology, an Army University Affiliated Research Center (UARC)

Current participants:

  • John Marken (PhD student, BE)
  • Andrey Shur (PhD student, BE)

Additional participants:

  • Rory Williams (PhD student, BE)

Collaborators:

Past participants:

Objectives

Aicb19-fieldprogrammable.png

This project explores the use of recombinases -- integrases, excisionases, and other methods of manipulating DNA -- as platform for engineering biomolecular circuits. Our high-level goal is to develop a design-oriented framework for recombinase-based, genetically-encoded circuits that can be used for detection, diagnostics, and logging of environmental signals and events. The specific objectives this project are to:

  • Develop circuit components, mathematical models, experimental protocols, and system characterization methods that enable recombinase-based circuits to be designed and utilized in a systematic fashion.
  • Implement a set of biological “event detectors” in both cell-free and cell-based assays, and demonstrate their utility in applications that combine digital, analog, and stochastic operations.

References



This research is supported by the Institute for Collaborative Biotechnologies through cooperative agreement W911NF-19-2-0026 from the U.S. Army Research Office. The content of the information on this page does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

  • Agency: Army Research Office
  • Grant number: W911NF-19-2-0026
  • Start date: 1 Dec 2018
  • End date: 30 Nov 2021
  • Support: 1 graduate student, part-time technician
  • Reporting: Annual reports