Difference between revisions of "Design and implementation of a synthetic biomolecular concentration tracker"

From MurrayWiki
Jump to: navigation, search
Line 2: Line 2:
 
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
 
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
 
| title = Design and implementation of a biomolecular concentration tracker
 
| title = Design and implementation of a biomolecular concentration tracker
| source = ACS Synthetic Biology (DOI 10.1021/sb500024b), (preprint posted on BioRxiv 10.1101/000448, 15 Nov 2013)
+
| source = ACS Synthetic Biology (DOI 10.1021/sb500024b)
 
| year = 2015
 
| year = 2015
 
| type = ACS Synthetic Biology article
 
| type = ACS Synthetic Biology article
Line 14: Line 14:
 
| id = 2015n
 
| id = 2015n
 
}}
 
}}
 +
 +
'''Preprints'''
 +
 +
BioRxiv DOI: [http://dx.doi.org/10.1101/000448| 10.1101/000448]
 +
 +
Posted: 15 Nov 2013

Revision as of 23:25, 17 May 2016


Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
ACS Synthetic Biology (DOI 10.1021/sb500024b)

As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.


Preprints

BioRxiv DOI: 10.1101/000448

Posted: 15 Nov 2013