Difference between revisions of "Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration"

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{{HTDB paper
 
{{HTDB paper
| authors = Emmanuel L. C. de los Santos, Victoria Hsiao and Richard M. Murray
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| authors = Emmanuel L. C. de los Santos*, Victoria Hsiao* and Richard M. Murray
 
| title = Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration
 
| title = Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration
| source = 2013 American Control Conference (ACC)
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| source = Presented, 2013 American Control Conference (ACC)
 
| year = 2012
 
| year = 2012
 
| type = Conference Paper
 
| type = Conference Paper
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| url = http://www.cds.caltech.edu/~murray/preprints/dhm13-acc.pdf
 
| url = http://www.cds.caltech.edu/~murray/preprints/dhm13-acc.pdf
 
| abstract =  
 
| abstract =  
This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task.
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This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in ''E. coli'' using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task.
 
| flags =  
 
| flags =  
 
| filetype = PDF
 
| filetype = PDF

Revision as of 19:44, 17 May 2016


Emmanuel L. C. de los Santos*, Victoria Hsiao* and Richard M. Murray
Presented, 2013 American Control Conference (ACC)

This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task.