Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli

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Title Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli
Authors Rory L. Williams, Richard M. Murray
Source 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
Abstract Application of synthetic biology is limited by the capacity of cells to faithfully execute burdensome engineered functions in the face of Darwinian evolution. Division of labor, both metabolic and reproductive, are underutilized in confronting this barrier. To address this, we developed a serine-integrase based differentiation circuit that allows control of the population composition through tuning of the differentiation rate and number of cell divisions differentiated cells can undergo. We applied this system to T7 RNAP-driven expression of a fluorescent protein, and demonstrate both increased duration of circuit function and total production for high burden expression. While T7 expression systems are typically used for high-level short-term expression, this system enables longer duration production, and could be readily applied to burdensome or toxic products not readily produced in bacteria.
Type Conference paper
URL https://www.biorxiv.org/content/10.1101/614529v1
Tag WM19-seed
ID 2019b
Funding ICB Microbial
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