Browse wiki
From MurrayWiki
Single day construction of multi-gene circuits with 3G assembly |
Abstract |
The ability to rapidly design, build, and … The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems take different approaches to accelerate DNA construction. We introduce a hybrid method, Golden Gate-Gibson (3G), that takes advantage of modular part libraries introduced by type IIS restriction enzyme systems and isothermal assembly's ability to build large DNA constructs in single pot reactions. Our method is highly efficient and rapid, facilitating construction of entire multi-gene circuits in a single day. Additionally, 3G allows generation of variant libraries enabling efficient screening of different possible circuit constructions. We characterize the efficiency and accuracy of 3G assembly for various construct sizes, and demonstrate 3G by characterizing variants of an inducible cell-lysis circuit. riants of an inducible cell-lysis circuit. +
|
---|---|
Authors | Andrew D Halleran, Anandh Swaminathan, Richard M Murray + |
Funding | Robust Multi-Layer Control Systems for Cooperative Cellular Behaviors + |
ID | 2018a + |
Source | 2018 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference + |
Tag | hsm18-seed + |
Title | Single day construction of multi-gene circuits with 3G assembly + |
Type | Conference paper + |
Categories | Papers |
Modification date This property is a special property in this wiki.
|
19 June 2018 05:10:34 + |
URL This property is a special property in this wiki.
|
https://www.biorxiv.org/content/early/2018/02/10/260851 + |
hide properties that link here |
Single day construction of multi-gene circuits with 3G assembly + | Title |
---|