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Cell-free and in vivo characterization of Lux, Las, and Rpa quorum activation systems in E. coli
Abstract Synthetic biologists have turned towards q
Synthetic biologists have turned towards quorum systems as a path for building sophisticated microbial consortia that exhibit group decision making. Currently, however, even the most complex consortium circuits rely on only one or two quorum sensing systems, greatly restricting the available design space. High-throughput characterization of available quorum sensing systems is useful for finding compatible sets of systems that are suitable for a defined circuit architecture. Recently, cell-free systems have gained popularity as a test-bed for rapid prototyping of genetic circuitry. We take advantage of the transcription-translation cell-free system to characterize three commonly used Lux-type quorum activators, Lux, Las, and Rpa. We then compare the cell-free characterization to results obtained in vivo. We find significant genetic crosstalk in both the Las and Rpa systems and substantial signal crosstalk in Lux activation. We show that cell-free characterization predicts crosstalk observed in vivo.
ation predicts crosstalk observed in vivo.  +
Authors Andrew Halleran, Richard M. Murray  +
Flags Biocircuits  +
Funding Robust Multi-Layer Control Systems for Cooperative Cellular Behaviors +
ID 2017g  +
Source ''ACS Synthetic Biology'', 7(2):752–755, 2017  +
Tag HM17-biorxiv  +
Title Cell-free and in vivo characterization of Lux, Las, and Rpa quorum activation systems in E. coli +
Type Journal paper  +
Categories Papers
Modification date
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26 January 2019 15:54:55  +
URL
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http://www.biorxiv.org/content/early/2017/07/07/159988  +
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Cell-free and in vivo characterization of Lux, Las, and Rpa quorum activation systems in E. coli + Title
 

 

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