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Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
Abstract We develop a system for implementing “pack
We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.
trains of a population in a defined order.  +
Authors John P. Marken, Richard M. Murray  +
Funding Field-Programmable, Recombinase-Based Biomolecular Circuits +
ID 2019c  +
Source qBio 2019 Conference  +
Tag MM19-qbio  +
Title Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation +
Type Conference paper  +
Categories Papers
Modification date
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9 June 2019 23:47:25  +
URL
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https://www.biorxiv.org/content/10.1101/591552v1  +
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Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation + Title
 

 

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