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Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation |
Abstract |
We develop a system for implementing “pack … We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order. trains of a population in a defined order. +
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Authors | John P. Marken, Richard M. Murray + |
Funding | Field-Programmable, Recombinase-Based Biomolecular Circuits + |
ID | 2019c + |
Source | qBio 2019 Conference + |
Tag | MM19-qbio + |
Title | Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation + |
Type | Conference paper + |
Categories | Papers |
Modification date This property is a special property in this wiki.
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9 June 2019 23:47:25 + |
URL This property is a special property in this wiki.
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https://www.biorxiv.org/content/10.1101/591552v1 + |
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Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation + | Title |
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