Field-Programmable, Recombinase-Based Biomolecular Circuits
This project funded by the Army's Institute for Collaborative Biotechnology, an Army University Affiliated Research Center (UARC)
This project explores the use of recombinases -- integrases, excisionases, and other methods of manipulating DNA -- as platform for engineering biomolecular circuits. Our high-level goal is to develop a design-oriented framework for recombinase-based, genetically-encoded circuits that can be used for detection, diagnostics, and logging of environmental signals and events. The specific objectives this project are to:
- Develop circuit components, mathematical models, experimental protocols, and system characterization methods that enable recombinase-based circuits to be designed and utilized in a systematic fashion.
- Implement a set of biological “event detectors” in both cell-free and cell-based assays, and demonstrate their utility in applications that combine digital, analog, and stochastic operations.
- Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation (John P. Marken, Richard M. Murray, qBio 2019 Conference)
- Proof of concept continuous event logging in living cells (Andrey Shur, Richard M. Murray, 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference)