BE 262 project, 2010

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This page contains a description of the BE 262 (bootcamp) project for 2010.

Project description

Equipment and supplies

Equipment

  • Basic cloning equipment: PCR machine, gel rig, pipettes, etc
  • Plate reader: can use Victor X3 from 040 Keck
  • Flow cytometer: plan to use HHMI-refurbished MoFlo cytometer
    • Talked to Dave Tirrell on 16 Aug about using this instrument. Need to arrange for training (RMM)
    • Need to figure out what materials (if any) need to be ordered to support likely experiments
    • Outside chance that the new BD FACS Calibur unit will arrive in time to try it out
  • (Optional) Fluorescent microscope for single cell imaging. Need moveable stage + autofocus to be able to look at several pads.

Supplies

  • Vectors: ???
    • Can we use current vectors that Oskar is testing? What are they?

Project setup

The following tasks need to be done before bootcamp to make sure that everything is ready for the project: