Difference between revisions of "Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation"

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|Title=Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
 
|Title=Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
 
|Authors=John P. Marken,  Richard M. Murray
 
|Authors=John P. Marken,  Richard M. Murray
|Source=Submitted, 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
+
|Source=2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
 
|Abstract=We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.
 
|Abstract=We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.
 
|URL=https://www.biorxiv.org/content/10.1101/591552v1
 
|URL=https://www.biorxiv.org/content/10.1101/591552v1

Revision as of 23:45, 9 June 2019

Title Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
Authors John P. Marken, Richard M. Murray
Source 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
Abstract We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.
Type Conference paper
URL https://www.biorxiv.org/content/10.1101/591552v1
Tag MM19-seed
ID 2019b
Funding ICB Network19
Flags