Biomolecular resource utilization in elementary cell-free gene circuits
Dan Siegal-Gaskins, Vincent Noireaux, and Richard M. Murray
To appear, 2013 American Control Conference (ACC)
We present a detailed dynamical model of the behavior of
transcription-translation circuits in vitro that makes explicit the roles
played by essential molecular resources. A set of simple two-gene test
circuits operating in a cell-free biochemical ‘breadboard’ validate this
model and highlight the consequences of limited resource availability. In
particular, we are able to confirm the existence of biomolecular ‘crosstalk’
and isolate its individual sources. The implications of crosstalk for
biomolecular circuit design and function are discussed.
Conference Paper
(PDF, 368K)
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Richard Murray
(murray@cds. caltech.edu)