Slide 45 of 79
Notes:
The last part of the talk deals with the experimental predictions that can be made from this research. Let us first look at how one could vary the rate constant for cyclin synthesis in an extract. Fertilized frog eggs are put into a tube and centrifuged. This breaks up the eggs and separates the cytoplasm. Because the extract contains maternal cyclin mRNA (mRNA from the mother) as well as the other major proteins and enzymes the extracts begins oscillating with roughly a 60 minute period. RNase added to the extract blocks mRNA and therefore the extract arrests in interphase because after cyclin is degraded the is no way it can create more. RNase inhibitor is added which destroys the RNase. Exogenous mRNA can be added to the extract in differing amounts giving experimentalists the ability to control the amount of cyclin synthesized.
